Antibiogram and heavy metal resistance pattern of Aeromonas hydrophila isolated from Guppy, Poecilla reticulata, ornamental fish from aquarium shop

Abstract

This study was carried out to investigate the mass mortality in ornamental fish Guppy, Poecilia reticulata, infected Motile Aeromonad Septicemia (MAS) due to Aeromonas hydrophila from ornamental fish aquarium shop in Tanah Merah, Kelantan, Malaysia with emphasis the bacterial isolates antibiogram and heavy metal resistance pattern. MAS due to A. hydrophila is an important disease to both human and aquatic animals. Mass mortality of MAS infection among ornamental fish lead to significant economic lose. Hence, there is a must to find out solution to overcome the arise problem. In the present study, a total of 100 bacterial isolates was successfully isolated and identified from diseased Guppy fish by using Glutamate Starch Pseudomonad (GSP) agar media. The bacterial isolates were then subjected to antimicrobial agent sensitivity test and heavy metal resistance assay by using disc diffusion and two fold dilution method, respectively. The findings of the present study showed Oxolinic acid was found can be effectively control the present bacterial isolates. High heavy metal resistance pattern and MAR index revealed that the sampled fish were highly exposed to the tested antimicrobial agent and heavy metals.